C6 peptide enzyme immunoassay in Lyme borreliosis serology

The cut-off values used in C6 peptide-based enzyme immunoassay (EIA), a widely test Lyme borreliosis (LB) serology, have not been thoroughly analysed. objective of the study was to examine performance EIA, and determine optimal for test. analysed data contained results 1368 serum samples. EIA index were compared statistically with immunoblot (IB) results. identified further tested well-defined LB patient cohort. Cut-off value 1.6 appeared be when as stand-alone When using first-tier test, 0.9 2.4 negative positive second-tier samples yielding ≥3.0 could considered positive. had also high sensitivity identify seropositivity among definite patients. refine role serology. Importantly, use leads reduction number that need an IB, thus reducing costs. Two alternative workflows serology including are suggested.